This article aims to investigate the impact of government regulation on the business innovation by analysing the case of Korean manufacturing companies. The analytical sample is gathered from the 2012 Korean Innovation Survey data of Korean Institute of Science & Technology Evaluation and Planning and merged with Korean Regulation Index data of Korean Institute of Public Administration. The analysis results show that the technical innovation of manufacturing firms is negatively associated with regulation stringency of manufacturing business. More specifically, the higher level of the manufacturing field’s regulation flow as well as regulation stock may hinder the firm’s innovation activity and performance. The results suggest the importance of regulation reform for promoting innovation of Science & Technology and business.